Cell-based kinase signaling pathway LinkLight™ assays utilize kinase-protein interactions as functional signal readouts. Kinases do no function alone, they interact with other proteins for proper function and signal transduction. Existing kinase assay technologies are mostly based on phosphorylation (or phosphorylation status) and omit the signaling networks which include signal cross-talking and feedback loops. The kinase signaling pathways are characterized by multiple kinase effector and signal adaptor interaction networks. LinkLight™ technology is utilized to monitor kinase/effector or signal adaptor interactions within living cells, preserving the complexity of the signaling pathways. Although the cell-based kinase LinkLight™ assays monitor specific kinase/effector or adaptor interactions as signaling pathway readouts, the assays also have characteristics of phenotypic screens which include unknown mechanisms modulating or regulating the interactions.
Cell-base kinase signaling pathway LinkLight™ (U2-OS cells if not specified) cells lines.
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Kinase LinkLight™ Cells
Description ALIAS Catalog
ERK2/β-arrestin LinkLight™ Cells MAPK1 5001-S
RAF1/β-arrestin LinkLight™ Cells c-Raf 5002-S
RAF1/14-3-3 LinkLight™ Cells c-Raf 5003-S
Src/β-arrestin LinkLight™ Cells c-Src 5004-S
ASK1/β-arrestin LinkLight™ Cells MAP3K5 5006-S (CHO)
ASK1/14-3-3 LinkLight™ Cells MAP3K5 5011-S
BTK/β-arrestin LinkLight™ Cells ATK, BPK 5012-S
GSK3β/β-arrestin LinkLight™ Cells SGKL 5020-S
ASK1/β-arrestin LinkLight™ Cells MAP3K5 5026-S (U2OS)